Part:BBa_K3962354:Design

Inducible expression of antitoxin MazE by pBAD

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  Illegal BamHI site found at 1283
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961
  

Design Notes

pBAD has been intensively investigated by previous iGEM projects and has tight regulation upon downstream genes. It is a suitable promoter for regulating antitoxin genes because pBAD can induce very high levels of MazE expression to neutralize toxic effects of MazE. Codon optimization was performed for the expression of the gene in E. coli.

Source

All sequences of subparts are from iGEM registry.